Intranigral Administration of β-Sitosterol-β-D-Glucoside Elicits Neurotoxic A1 Astrocyte Reactivity and Chronic Neuroinflammation in the Rat Substantia Nigra

Figure 6

Colocalization of S100β and GFAP immunoreactivity after a BSSG injection in the SNpc. (a) Representative micrographs of the double immunofluorescence in untreated (Ut) control rats and rats at different times (shown at the left side of micrographs) after BSSG injection. IFAD quantification for S100β (b) and GFAP (c) during the experimental time course. The S100β and GFAP values for the mock rats correspond to the quantification in Supplementary Figure 5. The values are the from four anatomical levels. independent rats in each time of each experimental condition. Two-way ANOVA and post hoc Bonferroni tests were applied for statistical analysis. The levels of significance were () and () compared with the DMSO mock and Ut groups of the respective immunostaining.

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