Intranigral Administration of β-Sitosterol-β-D-Glucoside Elicits Neurotoxic A1 Astrocyte Reactivity and Chronic Neuroinflammation in the Rat Substantia Nigra

Figure 7

Colocalization of C3 and GFAP immunoreactivities after a BSSG injection in the SNpc. (a) Representative micrographs of the double in untreated (Ut) control rats and rats at different times (shown at the left side of micrographs) after BSSG injection. Immunofluorescence area density (IFAD) for C3 (b) and GFAP (c) was determined using the ImageJ software v.1.46r (National Institutes of Health, Bethesda, MD). The C3 and GFAP values for the mock rats correspond to the quantification in Supplementary Figure 6. (d) Details of cells coexpressing C3 and GFAP on day 30 after a BSSG injection in the SNpc. The values are the from four anatomical levels. independent rats in each time of each experimental condition. Two-way ANOVA and post hoc Bonferroni tests were applied for statistical analysis. () indicates a compared with the DMSO mock and Ut groups of the respective immunostaining.

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