Intranigral Administration of β-Sitosterol-β-D-Glucoside Elicits Neurotoxic A1 Astrocyte Reactivity and Chronic Neuroinflammation in the Rat Substantia Nigra

Figure 9

Time course of leukocyte infiltration after a BSSG injection in the SNpc. (a) Representative micrographs of double immunofluorescence of TH and CD45 in untreated (Ut) control rats and rats at different times (shown at the left side of micrographs) after BSSG injection. IFAD quantification for TH and CD45 during the experimental time course. The (b) TH and (c) CD45 values for the mock rats correspond to the quantification in Supplementary Figure 7. The values are the from four anatomical levels. independent rats in each time of each experimental condition. Two-way ANOVA and post hoc Bonferroni tests were applied for statistical analysis. () indicates a compared with the DMSO mock and Ut groups of the respective immunostaining. (d) Representative photographs of brain slices after intravenous injection of the Evans blue dye on the days shown at the left of each row.

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