Signal Transduction in Astrocytes during Chronic or Acute Treatment with Drugs (SSRIs, Antibipolar Drugs, GABA-ergic Drugs, and Benzodiazepines) Ameliorating Mood Disorders

Table 1

Comparison between effects on gene expression and editing of chronic treatment with the SSRI fluoxetine or the antibipolar drug carbamazepine in cultured mouse astrocytes and in astrocytes freshly isolated from drug-treated mice.

Gene	Drug	FACS, astrocytes	Culture astrocytes
ADAR2	Fluoxetine	Up	Up
5-HT2B receptor expression	Fluoxetine	Up	Up
5-HT2B editing	Fluoxetine	Up	Up
5-HT2c receptor expression	Fluoxetine	Unchanged	Unchanged
cPLA2a	Fluoxetine	Up	Up
sPLA2	Fluoxetine	Unaltered	Unaltered
GluK2 expression	Fluoxetine	Up	Up
GluK2 editing	Fluoxetine	Up	Up
GluK4 expression	Fluoxetine	Unchanged	Unchanged
cfos expression	Fluoxetine	Up	Up
fosB expression	Fluoxetine	Up	Up
Cav 1.2	Fluoxetine	Up	Up
NBCe1	Carbamazepine	Up	Up
GluK2	Carbamazepine	Down	Down
cPLA2	Carbamazepine	Up	Up

Table 1 shows all experiments in which drug effects were compared in cultured astrocytes and in astrocytes freshly obtained by FACS as described by Lovatt et al. [3]. For FACS, astrocytes had been obtained from mice stained with GFP, based on expression of the astrocyte-specific GFAP, in transgenic animals and the stained cells were separated after cell dissociation by means of their fluorescent signal. The cultures were treated chronically with either fluoxetine or carbamazepine, and the animals had been treated chronically for a similar length of time (2 weeks). Complete agreement was found. From Peng et al. [2], with the exception of Ca_v1.2, which is from Du et al. [4], using similar techniques.